Silver Nanoparticles Functionalized by Fluorescein Isothiocyanate or Rhodamine B Isothiocyanate: Fluorescent and Plasmonic Materials

Stability of bovine serum albumin labelled by rhodamine B isothiocyanate

The aim of this study was to label Bovine Serum Albumin (BSA) by Rhodamine B Isothiocyanate (RBITC) and test its stability in vivo. RBITC-BSA was labelled by the improved Marshall’s method, scanned at full wavelength, and the RBITC:BSA ratio was calculated. Blood and urine samples were obtained from rabbits injected with the RBITC-BSA complex and SDS-PAGE analysis was performed. Full wavelength...

Light emitting diode microscope illumination for green fluorescent protein or fluorescein isothiocyanate epifluorescence.

Honey-Induced Protein Stabilization as Studied by Fluorescein Isothiocyanate Fluorescence

Protein stabilizing potential of honey was studied on a model protein, bovine serum albumin (BSA), using extrinsic fluorescence of fluorescein isothiocyanate (FITC) as the probe. BSA was labelled with FITC using chemical coupling, and urea and thermal denaturation studies were performed on FITC-labelled BSA (FITC-BSA) both in the absence and presence of 10% and 20% (w/v) honey using FITC fluore...

Rhodamine B isothiocyanate-modified Ag nanoaggregates on dielectric beads: a novel surface-enhanced Raman scattering and fluorescent imaging material.

Rhodamine B isothiocyanate (RhBITC) is a prototype dye molecule that is widely used as a fluorescent tag in a variety of biological applications. We report in this work that once RhBITC is adsorbed onto Ag on silica or polystyrene beads, it exhibits not only a strong surface-enhanced Raman scattering (SERS) signal but also a measurable amount of fluorescence. The RhBITC-modified Ag-deposited si...

Magnetic gold nanoparticles as a vehicle for fluorescein isothiocyanate and DNA delivery into plant cells

Magnetic gold nanoparticles (mGNPs) with uniform size and morphology synthesized by our sonication treatment method were covalently bound with fluorescein isothiocyanate (FITC) molecules. Driven by an external magnetic field, FITClabelled nanoparticles were delivered into plant cells with and without cell walls, evident from sectional transmission electron microscopy images. Confocal images fur...